The UV-Vis absorption spectra have to produce maximum absorbance values that describe the intensity of anthocyanin spectra in different colors for different pH. Clearly, the spectrum shows its pH dependency. The UV-Vis absorption spectra have to produce maximum absorbance values that describe the intensity of anthocyanin spectra in different colors for different pH. So, 520 nm is the wavelength of light we need to measure when spectrophotometrically determining the concentration of anthocyanins in any sample. However, there is a problem. Thus, we can exploit the unique pH-dependency of the anthocyanin absorption spectrum to selectively determine anthocyanin concentration spectrophotometrically and eliminate the other interfering substances from the … As it can be seen from the shape of curves, the obtained absorption spectra are characteristic of anthocyanin pigments ] l-3,5,8,9,1 11. Anthocyanins occur in all tissues of higher plants, including leaves, stems, roots, flowers, and fruits. One of the key factors affecting the bioavailability of anthocyanins is their transport through the gut epithelium. The pH-dependent chemical reaction of anthocyanins and the anthocyanin absorption spectrum is shown in the figure to the right. In non-senescent leaves, strong anthocyanin absorption was found between 500 nm and 600 nm (with a 70–80 nm apparent bandwidth). Absorption Spectra of the Anthocyanin Pigment of Red Cabbage: a Natural Wide‐range pH Indicator Frederick T. Wolf Department of Biology, Vanderbilt University, Nashville, Tenn. Anthocyanins are more stable at low pH (acidic conditions) which gives a red pigment. Anthocyanin Anthocyanins are widely known as nutraceuticals and are a group of soluble vacuolar pigments, which are red, purple, or blue in color based on the pH of the micro environment. They were markedly difference from anthocyanin-chloride and “genuine anthocyanins”. measuring the reflectance from the thin-layer chromatography plates and the absorption of the liquid-phase pigment extracts, resulting in spectral data for each pigment from 200 to 1400nm at 1-nm intervals. From: Quality Control and Evaluation of Herbal Drugs, 2019 So Differences on absorption spectra between two anthocyanin complexes were in the position of maximum in the ultraviolet region and also in that of shoulder and minimum existing 420-475nm. Meanwhile, the higher the pH value of anthocyanin will provide color fading of the color blue. At a pH of 1.0, due to the positive charge on the C ring, anthocyanins strongly absorb light between 460 and 550 nm (long blue, cyan and green light) and have an absorption maximum of about 520nm . Anthocyanins have only one absorption maximum in the visible light range (400 nm - 700 nm), and that is at 520nm.